The cardiopulmonary physiology of dinosaurs--and especially of the long-necked sauropods, which grew much larger than any land animals before or since--should be inherently fascinating to anyone involved in respiratory care. What would the blood pressure be in an animal 12 m (40 ft) tall? How could airway resistance and dead space be overcome while breathing through a trachea 9 m (30 ft) long? The last decade has seen a dramatic increase in evidence bearing on these questions. Insight has come not only from new fossil discoveries but also from comparative studies of living species, clarification of evolutionary relationships, new evaluation techniques, computer modeling, and discoveries about the earth's ancient atmosphere. Pumping a vertical column of blood 8 m (26 ft) above the heart would probably require an arterial blood pressure > 600 mm Hg, and the implications of this for cardiac size and function have led to the proposal of several alternative cardiopulmonary designs. Diverse lines of evidence suggest that the giant sauropods were probably warm-blooded and metabolically active when young, but slowed their metabolism as they approached adult size, which diminished the load on the circulatory system. Circulatory considerations leave little doubt that the dinosaurs had 4-chambered hearts. Birds evolved from dinosaurs, and the avian-type air-sac respiratory system, which is more efficient than its mammalian counterpart, may hold the answer to the breathing problems posed by the sauropods' very long necks. Geochemical and other data indicate that, at the time the dinosaurs first appeared, the atmospheric oxygen concentration was only about half of what it is today, and development of the avian-type respiratory system may have been key in the dinosaurs' evolutionary success, enabling them to out-compete the mammals and dominate the land for 150 million years. Key words: dinosaurs, sauropods, circulation, blood pressure, respiration, evolution, comparative physiology, endothermy, avian respiration, air sacs, dead space, trachea, gas exchange.
Full Text :COPYRIGHT 2009 Daedalus Enterprises, Inc.
Introduction: Why Dinosaurs?
How Can We Know Anything About the Physiology of Dinosaurs?
Circulation: How Could the Tallest Dinosaurs Have Perfused Their
Systemic Blood Pressures in the Largest Sauropods
Warm-Blooded, Cold-Blooded, or Both?
Structure of the Dinosaur Heart
Respiration: How Could They Have Breathed Through Such Long Necks?
Lessons From the Giraffe
How Birds Breathe
Implications of the Avian System With Respect to Tracheal Length
The Sauropod Respiratory System: Current Concepts
Influence of the Ancient Atmosphere
ConclusionsIntroduction: Why Dinosaurs?
Being asked to present the 35th Donald F Egan Memorial Lecture is a distinct honor, considering not only Dr Egan's contributions to education and clinical excellence in the field of respiratory care, but also the eminence of the previous presenters. I was thus deeply honored by the Program Committee's invitation, although their request that the topic be the physiology of dinosaurs initially gave me pause. This lecture typically acknowledges the career contributions of a leading researcher or clinician whose work has influenced respiratory care both as a field of endeavor and as a profession. It therefore usually consists of a review of those contributions in the context of the subject area in which the presenter has worked. That description would not apply this year if my topic was truly to be the cardiopulmonary physiology of dinosaurs. On reflection, though, I understood the Committee's intent. Several of its members knew of my longstanding involvement in the teaching of comparative respiratory physiology, (1-8) and of my interest in the largest dinosaurs as challenges to known physiologic principles and limits. In this context the assignment fits with Dr Egan's role in education--in expanding the scope of respiratory care, and in both broadening and stimulating the interests of its practitioners--and also with the purposes of the lectureship that honors him.
No members of the animal kingdom are as often or as prominently in the public eye as dinosaurs. (9) Whether in books, movies, the comics, video games, or advertisements in media of every type, dinosaurs remain more or less continually in our consciousness. This applies both to children--many of whom can reel off the names of a dozen different dinosaurs before they can name the street on which they live (9)--and to adults, thanks to the Discovery Channel, National Geographic, and other popular media. A Google Books search under "dinosaurs" in March 2009 retrieved nearly 5,000 citations, and there are currently several excellent books on dinosaurs for educated lay readers as well as for scientists working in this field. (10-12) And the study of dinosaurs is as active as any field of scientific endeavor; seldom does a month pass without a news report about some new discovery or insight into dinosaurs and the world in which they lived.
Given their size, their status as real-life "monsters," and their mysteriousness--having been extinct for 65 million years--it is no wonder that dinosaurs continue to fascinate many of us throughout our lives. However, for those of us involved in health care there are additional motivations for such interest. Respiratory therapists, nurses, physicians, and other health-care professionals are all biologists, in the sense that their work requires knowledge of the laws of the natural world and of the structure and function of living things. Respiratory care, especially, relies heavily on an understanding of physiology, and the areas in which the relevance of that understanding is most apparent are respiration and the circulation. It is also in these areas that the physiology of dinosaurs is most intriguing, particularly when one considers their physical dimensions.
Figure 1 illustrates the relative sizes of 2 well-known dinosaurs, Brachiosaurus and Mamenchisaurus. Given our understanding of the physiology of humans and other living animals, the implications of the sheer size of these creatures with respect to circulatory and respiratory function are truly staggering: what would the systemic blood pressure have to be in an animal standing 40 feet tall, and how could a creature breathe through a 30-foot neck? I will consider these questions in the context of present-day knowledge about dinosaurs and the physiology of different living animal groups, from the perspective of a clinician-educator interested in cardiorespiratory function.
How Can We Know Anything About the Physiology of Dinosaurs?
That we could know anything for certain about the physiology of dinosaurs seems at first a dubious proposition, given that physiology deals with soft tissues and organ function that can be observed, and that such knowledge would have to be generated many millions of years after the last dinosaur died. To place the challenge of studying dinosaur physiology into perspective, it is helpful to consider when in life's history they lived. (13) Geologists divide earth history into eons-extremely long intervals of time--and subdivide the eons into eras, which are in turn broken down into periods (Fig. 2).14 Periods are subdivided into epochs (eg, late, middle, and early), and these epochs are further divided into ages. The oldest rocks that survive to the present day are about 4 billion (4,000 million) years old, and the oldest fossils date from roughly that time. Animals with sophisticated internal or external skeletons first appeared about 570 million years ago, at the beginning of the Phanerozoic eon, which continues today. Dinosaurs lived during the Mesozoic era, which began approximately 250 million years ago and ended at the time of their disappearance, about 65 million years ago. The Mesozoic era is divided into the Triassic, Jurassic, and Cretaceous periods, which together lasted about 185 million years and can be thought of as the age of dinosaurs.
When I was first given the task of teaching comparative respiratory physiology to pulmonary fellows in the late 1970s, that field had essentially no overlap with paleontology. This was understandable, given that the latter was based on the study of the fossil record while the former relied on investigation of living animal species in the laboratory. A PubMed search for the word "dinosaur" retrieved only 11 citations to publications between 1965 and 1980, and only 1 when "dinosaur" and "physiology" were combined (Table 1). Somewhat more articles appeared during the 1980s, and more still during the 1990s. However, interest in dinosaur physiology among paleontologists and physiologists has mushroomed during the present decade, and PubMed returned 213 citations with the 2 search terms "dinosaurs" and "physiology" for the years 2000 through 2008. Table 1 gives only a partial view of the increased scientific activity in this interdisciplinary field; during the present decade, hundreds of other relevant articles have been published in paleontology, zoology, and earth-sciences journals, and other sources not included in Index Medicus.
While our knowledge of dinosaurs a half-century ago was based on the fossil record--primarily dinosaur bones--today the study of dinosaur physiology draws on many more information sources (Fig. 3). The fossil record is now much larger, thanks to new and better dinosaur discoveries on every continent, and to greatly expanded capabilities for studying them. Dinosaur fossils now include not only bones and teeth but also skin (and skin appendages, such as feathers) and perhaps even DNA (15) and soft tissues (16)--although these last two are controversial. The fossil record has yielded information not only about the dinosaurs themselves, but also about the plants they ate and other aspects of their environment. (17-21) The study of coprolites (fossilized feces) has generated an abundance of data about dinosaur diets and feeding habits. (19,22) Fossilized dinosaur eggs, some of them with embryos, (23-25) and well-preserved dinosaur nesting grounds (26,27) have greatly expanded our understanding of their reproduction and behavior.
Preserved footprints left in soft mud or clay as dinosaurs went about their daily lives, while not fossils in the usual sense, can provide information unavailable from the other sources described, and their study has become a whole subspecialty within dinosaur paleontology. (28-32) Some track-ways contain thousands of individual footprints made by dozens or even hundreds of individuals. From such sources can be derived insights not only into the dimensions and biomechanics of the animals that made them, but also about aspects of their lifestyles and behavior. For example, some trackways contain the footprints of large groups of herbivorous dinosaurs moving together across the landscape--and in a few cases also those of predators apparently stalking them.
Another area in which great advances have led to increased understanding of dinosaurs in general and also of their physiology is the study of evolutionary relationships among different groups of animals. (33-36) Several technical terms apply to this area, and not everyone working in the relevant fields agrees on how these should be used. Phylogeny refers to the evolution of genetically related groups of organisms, as distinguished from ontogeny, which is the development of the individual organism. Systematics is the scientific study of the diversity of organisms within and among genetically related groups, or clades. The related term taxonomy refers to the scientific practice and study of naming and ordering like groups of organisms. Systematics thus deals with understanding the relationships among different animal groups, and taxonomy deals with labeling them. Unfortunately, several approaches to the classification, description, and naming of individual species and groups of organisms are currently used, and this can make reading the relevant literature confusing. The approach used by most vertebrate paleontologists today is called phylogenetic systematics, or cladistics, which emphasizes the recency of common ancestry and the relationships among different clades. A cladogram is a graphical representation of the evolutionary relationships among groups of organisms. Figure 4 is a cladogram of our current understanding of the evolutionary relationships of dinosaurs, as constructed by Sereno. (37)
As technology has advanced in other areas of science and engineering, the application of emerging techniques to the study of dinosaurs has yielded important information about both recently discovered fossils and specimens already in museum collections. Paleontologists still do field work, and still spend time meticulously studying and describing specimens. However, they also rely heavily on mathematical and computer techniques, phylogenetics (the study of the relationships among organisms), taphonomy (the study of the process of fossilization), (38) paleoecology, (20) and many other techniques unavailable to the early dinosaur hunters. (39) Technologies familiar in health care, such as computed tomography and magnetic resonance imaging, have yielded important information about such previously inaccessible areas as the internal structures of fossilized animal bodies (40) and the contents of dinosaur eggs. (24) Increasingly sophisticated modeling techniques are being applied to the biomechanics and physiologic capabilities of the dinosaurs. (41-47)
As mentioned earlier, historically, comparative physiologists had little to do with the study of dinosaurs. However, this has changed in the last 20 years. During this time, numerous investigators have sought insight into the physiology and behavior of dinosaurs by comparison with living species, (48-56) especially with respect to respiration in birds. (53-56)
Current concepts of dinosaur physiology have also been influenced by an increased understanding of the history of the earth's atmosphere, particularly with respect to the concentration of oxygen ([O.sub.2]) in the air over geologic time. The atmosphere has not always had an [O.sub.2] concentration of 21% (160 mm Hg at sea level). Evidence of long-term change in the atmosphere's [O.sub.2] concentration has come mainly from the study of rocks, particularly as they reveal the geochemical cycles of carbon and sulfur during the last 550 million years. (57-59) Weathering of organic carbon and pyrite sulfur results in net [O.sub.2] consumption, whereas burial of organic carbon and pyrite sulfur in sediment (which indicates an excess of global photosynthesis over global respiration) results in net [O.sub.2] production. (59) Several computer models have been developed for estimating atmospheric [O.sub.2] (and C[O.sub.2]) at different times in earth's geologic history. These models employ different factors, such as the amount of reduced carbon and sulfur in sediment samples, the ratio of the carbon isotopes [sup.13]C and [sup.12]C, and the ratio of the sulfur isotopes [sup.34]S and [sup.32]S in rock samples. (58,60,61)
While the different models yield different estimates for [O.sub.2] and C[O.sub.2] concentrations during the last 200 million years, there is agreement that atmospheric [O.sub.2] has varied substantially during the Phanerozoic eon, with a maximum of 30-35% about 300 million years ago. Estimates of the nadir atmospheric [O.sub.2] concentration, approximately 200 million years ago, range from 10% (62,63) to 15%. (58,60) Later in this paper I will discuss how the much lower atmospheric [O.sub.2] concentration at the beginning of the age of dinosaurs may have influenced the evolution of their respiratory systems.
Circulation: How Could the Tallest Dinosaurs Have Perfused Their Brains?
The dinosaurs that raise the most fascinating questions with respect to circulatory physiology are the sauropods (64-66) (Fig. 4, upper-right quadrant), because this group includes the largest creatures ever to have lived on land. (67) Although there has been increasing evidence that the cardiovascular and respiratory systems of the sauropods and the theropods (Fig. 4, lower-right quadrant) were very similar, this discussion will concentrate on the sauropods, simply because of the hemodynamic implications of their enormous size and the fact that most of the existing data and published speculation relate to the sauropods.
Just how big some of the sauropods were is dramatically evident to anyone who has passed through Concourse B of the United Airlines terminal at Chicago's O'Hare Airport, where the Field Museum's renowned Brachiosaurus skeleton stands amid the throng of human travelers (Fig. 5). Stopping for a moment beneath the thorax of this monster, a traveler familiar with the blood pressures and other aspects of circulatory dynamics encountered in clinical medicine might well wonder how it could have been possible for this creature to pump blood all the way up to a head nearly 40 feet above the floor--and, if so, what the pressures involved would have to have been. These questions have intrigued both paleontologists and physiologists in recent decades, and, despite the impossibility of direct measurements, a number of lines of evidence (or at least reasoning) have been offered in the literature.
Systemic Blood Pressures in the Largest Sauropods
Of the 121 known sauropod species, (68) most were much bigger than terrestrial mammals, and at least a dozen were truly enormous--an order of magnitude larger than the ornithischian dinosaurs and any land mammal that ever lived. (68) Familiar species, such as the slender Diplodocus and stockier Apatosaurus ("Brontosaurus"), known from multiple well-studied specimens and thus of the most confidently known size, were 22-25 m (74-82 ft) in length, and Brachiosaurus stood as tall as 13 m (43 ft) (41,65) Although they are known only from very incomplete skeletons, several other, more recently described sauropod species can confidently be said to have been substantially larger, although precisely how large cannot be known until more complete specimens are found. Supersaurus, Seismosaurus, and Amphicoelias, all from the same fossil beds in the western United States, were similar to Diplodocus but larger, and may have reached lengths of 40 m (132 ft) and heights of 17 m (56 ft). (65,67) Another species, Paralititan, discovered more recently in Egypt, was a giant in the same size range. (69) Although body weights cannot be determined as precisely as lengths, the current estimates are that these largest sauropods weighed as much as 50-80 tons (67,70)--and perhaps even more in the case of Argentinosaurus, a member of the recently discovered Titanosauria from South America. (70)
By simple proportionality, the systemic arterial blood pressures in the tallest sauropods would have to have been several-fold greater than that of the giraffe, the tallest living mammal--at least if they stood erect, perfused their brains continuously, and had circulatory systems similar to those of living vertebrates (Table 2). Blood pressures in the giraffe have been measured in several studies, (71,72) and a systolic pressure in the carotid artery of 260-300 mm Hg has been taken as representative. Calculated blood pressures for the tallest sauropods, based on the height of the presumed column of blood that would have to be pumped to the brain, approach 1,000 mm Hg, a value that begs credibility in terms of the strength and pressure-generating capabilities of known animal tissues. Putting aside the largest giants, whose height is admittedly speculative, pressures in the well-studied Brachiosaurus, in which a standing height of at least 12 m is accepted, the pressures involved--well over 600 mm Hg (73)--are so much greater than any ever measured in living animals as to make one wonder whether a circulatory system like that of the giraffe would have been possible in such animals.
Discussions of the potential magnitude of sauropod blood pressure spilled over into the clinical literature soon after the American Museum of Natural History installed a new exhibit in its main atrium in 1991. This exhibit contains the mounted skeleton of an adult Barosaurus (a slender sauropod similar to Diplodocus), rearing up on hind legs to protect her calf from a menacing Allosaurus, a theropod predator related to Tyrannosaurus. The head of the adult Barosaurus in this dramatic display is 12 m above its thorax, prompting Choy and Altman at the investigative cardiology laboratory at St Luke's Roosevelt Hospital in New York to question the feasibility of the blood pressures that would have been required with a conventional mammalian-type cardiovascular system. (74) They estimated that Barosaurus would have had an arterial blood pressure of 880 mm Hg leaving the heart, and that its heart rate would have had to be very slow, which would mandate a series of one-way valves in its carotid arteries to prevent blood from falling back into the chest between contractions. In association with these valves, Choy and Altman further proposed that Barosaurus may have had several localized concentrations of muscular tissue--essentially a series of auxiliary hearts--to pump the blood up to the head (Fig. 6). (74) Each "heart" in this series, located 2.44 m below the one above, would therefore have to generate a pressure of only 200-250 mm Hg. Bakker (75) had previously speculated that the long-necked sauropods might have used cervical muscular contractions of some sort to augment circulation to their heads, but the construct of Choy and Altman was unique in suggesting that the augmentation was provided by the cardiovascular system itself.
Publication of Choy and Altman's multiple-hearts proposal in The Lancet (74) drew attention from the comparative-physiology community and was shortly followed by 5 letters to the editor. (76-80) These mostly disparaged the idea, and more than one subtly questioned whether medical researchers should be venturing into speculations about dinosaurs. After first pointing out technical mistakes in Choy and Altman's argument (eg, based on height, the Barosaurus blood pressure would have been 740 mm Hg, not 880 mm Hg; and valves on both sides of each auxiliary heart would have been unnecessary), and noting that multiple cervical vascular pumps might cause discontinuous blood flow to the brain and predispose the animal to a variety of injuries, Millard et al argued that because Barosaurus was most likely amphibious, its neck would have been supported by water most of the time. (76) This latter assertion had previously been made by others, (81) based on theoretical calculations of sauropod hemodynamics in the erect posture.
Choy and Altman speculated that Barosaurus would be subject to frequent fainting as it moved from the head-down to the head-up position. (74) Rewell (77) expressed doubt about this, pointing out that if a standing giraffe were to lose consciousness, it would probably sustain a spectacular cervical dislocation. In a letter published the following month, Dennis (78) supported the multiple-hearts hypothesis, and agreed with Rewell's observation about the unlikelihood of frequent syncope in sauropods. Dennis also calculated that, according to the law of Laplace (which says that the pressure generated by the heart is directly proportional to the ventricular-wall thickness), in order to generate 740 mm Hg (per Millard et al), (76) Barosaurus would have had to have a ventricular wall 90 cm thick and a heart weighing 7.3 tons (78)--which he considered highly unlikely. Taylor (79) took issue with Millard's proposal that Barosaurus was semi-aquatic, pointing out several structural features in favor of a terrestrial lifestyle and doubting that it could have breathed snorkel-fashion while submerged.
Hicks and Badeer wrote that multiple hearts would not have been necessary, because circulation in the neck of Barosaurus most likely functioned as a siphon. (80) These investigators noted that fluid flow in a siphon depends not on the height of the loop but on the relative levels of its 2 ends, as well as on overcoming the resistance of the system. Hicks and Badeer had previously published a study on circulation in the giraffe, (82) and subsequently elaborated on the siphon hypothesis as applied both to giraffe (83) and to Barosaurus. (84) However, Dennis (78) noted that the pressure at the top of a siphon tends to be sub-atmospheric, and argued that the net flow of blood out of the carotid artery and into the brain would be impossible under such circumstances, as was pointed out subsequently by others. (85)
Another way the sauropods might have avoided having to generate extremely high arterial pressure is not to have raised their heads much above the horizontal position. This possibility was mentioned in passing by Seymour in 1976, (81) and has since been explored by several investigators. In fact, many sauropods may not have been capable of raising their necks to the full vertical position. Stevens and Parrish (86) used an interactive software package to construct and manipulate detailed 3-dimensional digital models of Diplodocus and Apatosaurus, and attempted to reconstruct the limits of deflection attainable with each neck vertebra and hence the range of motion and likely posture assumed by these dinosaurs during life. They found that the necks of both genera were straight (horizontal) in the neutral position, and that neither had as much flexibility (particularly for raising the head) as has been depicted in many reconstructions. These investigators concluded that the maximum feeding height for Diplodocus would have been about 4 m, barely above the level of its back; maximum dorsiflexion in Apatosaurus was somewhat greater, with an upper feeding height of approximately 6 m. Thus, "rather than flexing their necks like dinosaurian counterparts of giraffes or swans, [Diplodocus and Apatosaurus] appear to have fed more like giant, longer-necked bovids [such as cattle]." (86)
From other computer-modeling studies, those same investigators concluded that Brachiosaurus also held its head closer to horizontal than to vertical, and had a maximum browsing height similar to that of Apatosaurus (about 6 m). (43) Recent work with ostriches, which combined dissection of fresh neck specimens, experiments with neck vertebrae, photography of living birds, and computer modeling, supports the findings of Stevens and Parrish: studies that used fossilized neck vertebrae of Diplodocus carnegii and compared those data to data from ostriches indicated that the sauropod's neck positions of rest and maximum dorsiflexion were considerably more restricted than those of the ostrich. (52)
Perhaps, even if the sauropods were able to raise their heads to the heights assumed in Table 2, they did so only intermittently and did not perfuse their brains continuously as they did so. Given that a loss of cerebral blood flow during cardiac arrest in humans results in brain injury within a very few minutes, followed by death if it is not quickly restored, it seems counterintuitive to propose that animals as active as the giant sauropods could have survived with only intermittent perfusion to the head. However, recent evidence from several areas of investigation supports the concept that several vertebrate groups exhibit remarkable tolerance of brain hypoxia, (87-89) and it is possible that multiple adaptations protected the sauropod brain against anoxic injury.
Some turtles, amphibians, and fishes can survive in virtual anoxia for prolonged periods. (90,91) Certain seals and other diving mammals can withstand dramatically reduced brain-oxygen access for nearly an hour, while remaining active and engaging in complex behaviors. (91) The recently discovered oxygen-carrying molecules neuroglobin and cytoglobin, which appear to be widely distributed in vertebrates and of ancient origin, may play important roles in cerebral oxygen metabolism and hence in neuroprotection from anoxic injury. (93-95) Maybe sauropod brains were richly supplied with neuroglobin and also with metabolic protections against anoxia. Combined with microcirculatory adaptations, these protections enabled them to raise their heads high for periods long enough to scout their surroundings, fight, mate, and engage in other necessary activities despite reduced or even temporarily interrupted cerebral perfusion.
Table 3 summarizes the pros and cons of the various proposed mechanisms of circulation in the giant sauropods.
Warm-Blooded, Cold-Blooded, or Both?
Historically, dinosaurs were imagined as much larger versions of present-day reptiles: cold-blooded and sluggish. Although it had been postulated earlier, the notion that they may have been warm-blooded, and thus much more active than originally believed, became widely discussed following publication of a paper in Nature by Bakker in 1972. (96) Bakker provided several kinds of evidence to support this hypothesis. As demonstrated by their footprints, dinosaurs walked with fully-erect gaits and a narrow trackway, like modern mammals but unlike living reptiles. Dinosaurs had joint anatomy more like mammals than reptiles, and were probably capable of running much faster than was originally assumed. Certain aspects of dinosaur bone histology resemble those of mammals more than those of living reptiles. In addition, in several large assemblages of fossil dinosaurs of multiple species preserved together, the relative prevalence of carnivores in relation to herbivores (the predator-to-prey ratio, which is an indicator of the metabolic needs of the carnivores), is closer to that of mammals such as tigers than to their present-day reptilian counterparts such as crocodiles or Komodo dragons. Studies on the geographic distribution of dinosaurs indicate that they lived in temperate climates, and even sub-polar regions, where relying on external heat sources would seem infeasible.
The question of whether the giant sauropods were warm-blooded or cold-blooded has important implications with respect to their cardiovascular systems. Seymour and Lillywhite (85) revisited the issue of sauropod blood pressures in relation to how big their hearts must have been. They considered Barosaurus and assumed a blood pressure of 700 mm Hg, based on the length of its neck. They compared the known cardiac dimensions and hemodynamics of the fin whale, a warm-blooded mammal with a mass of 40 tons, to those projected for a Barosaurus of the same weight, assuming that its metabolism was similar to that of the whale. Table 4 summarizes that comparison, (85) which indicates that the sauropod would have to have had a heart 15 times heavier than a whale of the same body mass. Noting that the calculated dimensions of the Barosaurus heart were proportionally far greater than those of any living warm-blooded animals, the authors concluded that warm-bloodedness, as demonstrated by typical mammals, was most unlikely in Barosaurus (Fig. 7). (85) Instead they proposed that Barosaurus had an intermediate metabolic rate, less than that of typical warm-blooded animals, which would require substantially less cardiac muscle mass to sustain the same arterial blood pressure.
Warm-bloodedness and cold-bloodedness are easy concepts to comprehend when using familiar examples: my cat generates her own body heat, is continuously active, and maintains a constant body temperature of 38.5[degrees]C, whereas my pet tortoise is dependent on the temperature of the room, may be inactive for hours or days at a time, and feels cold to my touch. However, the situation with respect to the metabolic rates and body temperatures of animals is actually far more complex than this. The tuna, a fish and thus presumably "cold-blooded," is actually "warm-blooded," with a core body temperature substantially above that of the water it inhabits. (97) The situation with respect to metabolism and thermoregulation is particularly complicated as it pertains to the largest dinosaurs.
Rather than the single oversimplified construct of "warm-blooded" versus "cold-blooded," 3 different sets of terms are used to describe animals' metabolic state and temperature regulation (98):
1. Whether they can maintain a steady body temperature irrespective of temperature variations in their environment: they are said to be homoiothermic if they can, and poikilothermic if they cannot.
2. Whether they maintain their optimum activity temperature by means of internally generated heat: endothermic if they do, and ectothermic if they do not.
3. Whether their internal body metabolism operates at a high or low rate: tachymetabolic if it is high, bradymetabolic if low.
These 3 aspects of body-temperature regulation may appear to vary independently in certain circumstances. For example, hummingbirds, extremely active endotherms with daytime body temperatures of nearly 41[degrees]C, undergo a substantial fall in body temperature at night, to conserve energy, whereas the largest crocodiles, which are ectothermic and generally bradymetabolic, maintain essentially constant body temperatures (that is, are virtually homoiothermic) because of the thermal inertia of their large body mass. (99)
Given their adult size, the largest sauropods must have grown at phenomenal rates. Their eggs were relatively small, (23) and to reach an adult weight of 80 tons a 10-kg hatchling would have to increase its mass by nearly 5 orders of magnitude. (67) Indeed, it has been estimated that Apatosaurus attained its adult weight of about 25,000 kg in as little as 15 years, with a maximum growth rate > 5,000 kg/y. (100) Sauropods grew at least as fast as any mammal--a conclusion that is supported by histologic studies of their bones, which revealed mammalian-type growth patterns. (98,101,102) Endothermy and tachymetabolism would be highly advantageous--if not a requirement--for such rapid growth.
However, the same endothermy and tachymetabolism that facilitated rapid growth in young sauropods would have posed major problems once they reached adulthood. (56.67) The concept of thermal inertia--the propensity of larger animals to retain heat, in comparison to smaller animals--has led some authors to discount the possibility of endothermy for the giant sauropods. On the basis of allometric calculations, given their need to dissipate heat generated by physical activity, Gillooly et al (103) predicted that sauropods that weighed 50 tons would be at the upper limit of body temperature compatible with life. Because of these considerations the concept has been put forth that the giant sauropods did not maintain a single metabolic rate throughout their lives, but instead were endothermic and tachymetabolic when they were young and growing rapidly, and then slowed their metabolic rate as they approached adult size. (56) This strategy would be compatible with the intermediate metabolism proposed by Seymour and Lillywhite for the adult Barosaurus (85) (Fig. 7), and would imply lower food requirements as well as the ability to avoid lethal hyperthermia in the largest sauropods. (56,67)
Structure of the Dinosaur Heart
Whether the giant sauropods were endothermic or ectothermic, and whether their systemic arterial blood pressure was 700 mm Hg or 200 mm Hg, one conclusion about their cardiovascular systems seems inescapable: they had to have had 4-chambered hearts. Reptiles such as lizards and snakes have 3-chambered hearts, with 2 atria and a single ventricle (Fig. 8a). (4) With one ventricle pumping blood into both the systemic and pulmonary circuits, the pressures in these 2 circuits cannot be very different. As a result, in order for the pulmonary circulation to facilitate efficient gas exchange through thin-walled capillaries, the pressure has to be kept low. Generating systemic pressure sufficient to deliver blood to a head held very much higher than the heart would also subject the pulmonary circulation to a pressure incompatible with its function--even if, as in birds, the pulmonary capillaries were stronger and better supported than those of mammals. (104) However, the 4-chambered hearts of birds and mammals (Fig. 8b) (4) not only facilitate more efficient oxygenation of the blood, via elimination of the right-to-left shunt, but also permit the coexistence of a high-pressure systemic circulation with a low-pressure pulmonary circulation. Another difference between living animals with 3-chambered versus 4-chambered hearts is that the former have 2 systemic aortas, whereas the latter have only one.
That a double-pump, 4-chambered heart would have been a necessity in large dinosaurs has been pointed out by a number of authors over the years. (105-107) However, no empirical evidence was at hand, given the unlikelihood of preservation of soft internal organs in the fossil record. This situation was altered in 2000, with the publication of what was apparently anatomic documentation of a 4-chambered dinosaur heart. (108) Examination of a concretion within the uncrushed thorax of a late-Cretaceous ornithischian dinosaur, using computed tomography scanning, revealed what appeared to be a heart with 2 ventricular cavities and a single systemic aorta. The predicted design for the dinosaurian heart thus appeared to have been confirmed. However, that the reported structures actually represented a dinosaur heart was subsequently challenged, in this instance on both anatomic and geologic grounds. (40)
As with most other things in comparative physiology, the distinction between 3-chambered and 4-chambered hearts is not quite so straightforward as Figure 8 implies. Some reptiles have functional modifications that at least partially separate the systemic and pulmonary circulations, which permits a higher than expected systemic blood pressure. (42) However, regardless of the validity of the features claimed for the specific fossil in question, (108) the presence of many other avian features in dinosaurs, along with the inescapable hemodynamic challenges presented by the largest sauropods, would seem to guarantee that they had true 4-chambered hearts.
Although no live specimens are available for direct examination, evidence from the fossil record and inference from studies on living animals leads to several conclusions about circulatory function in dinosaurs, and especially in the giant sauropods:
* Their systemic blood pressure had to have been substantially higher than in living reptiles, and potentially higher than in any known creatures: 600 mm Hg and higher if they held their necks erect and perfused their heads in that position.
* Proposed alternative pumping arrangements, such as multiple auxiliary cervical hearts, would require less extreme blood pressure but are without known precedent.
* That the circulation in their necks may have operated as a siphon, which also necessitates the generation of lower blood pressure, is conceivable but doubtful because of the probable existence of negative intravascular pressure in the head.
* The enormous and probably unrealistic ventricular mass necessary to generate the predicted blood pressure would be much reduced if the sauropods were not "warm-blooded" in the conventional sense, but instead had lower metabolic rates when they were adults. This hypothesis is supported by bone histology and thermoregulatory evidence.
* They may not have held their necks erect, like giraffes, or only intermittently if they did so, and may have had adaptations that protected against anoxic injury during periods of reduced or absent cerebral perfusion.
* Their systemic and pulmonary circulations must have been separate, which mandates a double-pump, 4-chambered heart.
Respiration: How Could They Have Breathed Through Such Long Necks?
In addition to challenging our understanding of circulatory physiology, the huge size and especially the very long necks of the giant sauropods prompt the question, how did they breathe? Their long necks pose the most obvious challenge, with their inherent implications for airway resistance and dead space. But before discussing those factors it is necessary to take a brief detour and consider whether the sauropods could breathe underwater, snorkel-fashion, as was contended for many years.
When the first fossilized sauropod skeletons were discovered and attempts were made to reconstruct the animals that had left them, they were imagined as oversized versions of lizards and crocodiles: low to the ground, sluggish, and almost too heavy to move about. (68,107,109,110) They were commonly depicted partially or completely immersed in a swamp or lake, where the water would help to support their enormous bodies, and were assumed to have breathed snorkel-style, by raising their heads to the surface. This is the image of "Brontosaurus" (Apatosaurus) that I remember from books, magazines, and museum dioramas when I was a child in the 1950s. (111)
But they could not have breathed that way. Quite apart from the wealth of evidence that the sauropods were terrestrial animals, (17,64-66) some basic physical considerations quickly demonstrate that it would not have been physically possible (Fig. 9). (79,107,112,113) If a sauropod such as Barosaurus stood under water and extended its neck to the surface to breathe, the vertical distance from mouth to lungs might be 10 m. The pressure exerted by water at a depth of 10 m (2 atmospheres [1,520 mm Hg, or 2,068 cm [H.sub.2]O]) is twice that at the water's surface (1 atmosphere [760 mm Hg; 1,034 cm [H.sub.2]O]). Maximum inspiratory pressure in healthy adult humans is not much more than 100 cm [H.sub.2]O, (114) and only a small proportion of that is normally needed, even with strenuous exertion. It is difficult to imagine a dinosaur generating inspiratory pressures 10 times that for prolonged periods.
Not only would the sauropod in Figure 9 have been unable to expand its chest to inhale, but once it attempted to do so, the pressure difference between the pulmonary capillaries and alveoli would result in immediate hemorrhage and airway flooding. Intravascular and airway pressures would increase in tandem as the external water pressure increased, so that the difference between them would remain unchanged, until the sauropod opened its mouth to breathe. At that point, though, the pressure in the airway would fall to atmospheric (zero, relative to the pressure at the water's surface) and expose the pulmonary circulation's thin gas-exchange membrane to a pressure drop far exceeding what it could withstand.
There is another reason the scenario depicted in Figure 9 could not work. As will be discussed, both the probable design of the sauropod respiratory system and accumulating evidence from sauropod bones indicate that sauropods were almost certainly lighter than water, not heavier.
Lessons From the Giraffe
One of the most intriguing questions about the giant sauropods is how they were able to breathe through such long necks. The necks--and presumably the tracheas--of some species were very long indeed. From a detailed examination of skeletons of the largest available sauropods, Wedel (115) provided the data shown in Table 5. Although some of the extreme neck lengths shown are highly speculative (and in the case of Amphicoeleas, based on a single neck vertebra that has since been lost), it is clear that these animals had necks (and tracheas) far longer than those of any living animal.
The problem with a very long trachea relates to the dual problems of airway resistance and dead space. Because resistance to air flow varies not only with the length of a tube but also inversely with the fourth power of its radius, small changes in airway caliber cause much larger changes in resistance and hence in the work of breathing. If its diameter were too small, airway resistance in the sauropod trachea would render breathing impossible. On the other hand, although a wider trachea would have much less airway resistance, its volume (which increases as a function of the square of the radius) and hence the anatomic dead space would increase such that the tidal volume required to maintain effective gas exchange would be prohibitive.
Insight into this problem can be gained from studies on the giraffe, the living animal with the longest neck. An adult male giraffe can reach a standing height of nearly 5.5 m (18 ft), and the trachea approaches 2.5 m (8 ft). Hugh-Jones and colleagues (116) made in vivo breathing measurements on a giraffe somewhat smaller than this from the London Zoo that required general anesthesia for an operation on its foot. The animal subsequently developed complications and had to be euthanized, so that the investigators were able to compare anatomic measurements with those they had made during life. The giraffe's in vivo respiratory rate was 8-10/min, with a tidal volume of 3.3 L and a dead-space fraction (ratio of dead space to tidal volume) of 0.30. At autopsy the trachea was 170 cm long and had an internal diameter of 3.8 cm and a dead space (calculated from the excised trachea) of 1.9 L.
Hugh-Jones et al compared the giraffe's minute ventilation to its weight, and to similar data from humans, as well as from the red deer, llama, and camel, and found that these had a constant relationship, which indicates that the giraffe's ventilatory demand and overall response were as expected. (116) However, when they compared the giraffe's tracheal dimensions to those of other large mammals (Fig. 10), (116) it was clear that the giraffe is an outlier; it has a longer, narrower trachea than the other species. These findings, along with their calculations that showed that the air flow during tidal breathing in the giraffe was such that turbulence would be induced if it were increased even moderately, are consistent with field observations that giraffes are not capable of more than short bursts of strenuous exertion.
It is hard to imagine that an animal with tracheal proportions similar to those of the giraffe could survive with a trachea several times longer. Either the resistance or the dead space volume--or both--would seem unrealistic if the animal had mammalian-type tidal ventilation. Yet sauropods with extremely long necks not only existed but apparently flourished, which suggests that the very long trachea was not a barrier to evolutionary success. The search for a solution to this conundrum leads us to the birds, which evolved from dinosaurs and have a respiratory system very different from that of the giraffe-one in which a very large dead space is of little concern.
How Birds Breathe
Unlike mammals, birds have no diaphragm, and their respiratory organs are not confined to the thorax (Fig. 11). (117-24) The respiratory apparatus includes a system of air sacs of various sizes, in the neck, thorax, and abdomen (Fig. 12). (125-127) These air sacs are connected to the lungs, and also to the bones of the axial skeleton and limbs, most of which are hollow and extensively invested by extensions of the air sacs. This latter phenomenon is called skeletal pneumaticity, and birds are the only living vertebrates in which it occurs caudal to the skull. Most birds have 9 air sacs: 1 interclavicular sac plus paired cervical, anterior thoracic, posterior thoracic, and abdominal sacs. (128) The air sacs are poorly vascularized and do not participate substantially in gas exchange. (129) The lungs themselves are relatively small, compact, and denser than their mammalian counterparts (Fig. 13). (119)
Instead of a branching bronchial tree into and out of which each breath must pass, the bird's lung consists of a richly vascularized system of parallel air tubes (parabronchi), through which the air moves in one direction (Fig. 14). (122) Thus, birds breathe very differently from mammals, with a very different path for ventilation and the participation of air from more than one breath in both ventilation and gas exchange at any one moment.
Figure 15 shows conceptually how this system works. (118,119,130) Special adaptations of the skeleton and musculature permit substantial changes in the volume of the abdomen, anterior thorax, and neck, which ventilates the air sacs in those areas, while the thorax remains relatively rigid in the region near the spine overlying the lung. The lung thus changes very little in volume during the phases of respiration, and air passes through the lung between the bellows-like air sacs, in a caudal-to-cranial direction. Air movement through the various parts of the system during inspiration and expiration (see Fig. 15) is accomplished without the use of physical valves. (131,132) Instead, a fluidic mechanism (aerodynamic valving) determines the direction of air flow, in at least some instances by means of a structural adaptation in the bronchial wall known as the segmentum accelerans (or endobronchial tumescence), in association with branch points to the secondary bronchi. (55,127,133,134) As an aside, a similar fluidic valving mechanism has been applied clinically in respiratory care, in the design of the Monaghan 225 ventilator, (135) which found application in electricity-free and hyperbaric environments, (136) and was also used in the design of other less successful devices. (137,138)
The efficiency of gas exchange in the avian lung is augmented by cross-current ventilation-perfusion matching (Fig. 16), (103,121,122) which results in greater oxygen extraction than in the mammalian system. In fact, the crosscurrent ventilation-perfusion pattern allows the alveolararterial oxygen difference ([MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII], as calculated with the alveolar gas equation in humans) sometimes to be negative. This greater gas-exchange efficiency is illustrated in Figure 17, (119) which depicts ventilation and perfusion in a bird flying at an altitude of 6,100 m (20,000 feet). The design of the avian respiratory system (along with other adaptations) (139,140) means that birds can engage in physical activity unattainable by mammals and function normally at altitudes at which mammals could not survive. (141) One example is the semiannual migration of the bar-headed goose (Anser indicus), over the Himalayan mountains, to altitudes of [greater than or equal to] 9,100 m (30,000 feet). (140,142) Another is the extreme feats of activity and endurance accomplished by migrating shore birds, such as the bar-tailed godwit (Limosa lapponica baurei). Flying is the most energetically costly form of locomotion, (143) but migrating bar-tailed godwits tracked with surgically implanted transmitters have recently been documented to fly non-stop as far as 11,680 km (7,242 mi) directly across the Pacific Ocean from Alaska to New Zealand, in flights of up to 9 days without rest, food, or water. (144)
Aspects of the structure of the gas-exchange interface, in addition to those inherent in flow-through ventilation and cross-current perfusion, probably help to explain the greater efficiency of avian lungs, compared to those of mammals. From the parallel parabronchi shown in Figure 14 there extend blind-ended terminal air spaces called air capillaries, which have a thinner alveolar-capillary membrane (145) and are also smaller and more densely packed than mammalian alveoli. (146,147) Recent studies by West and colleagues on the lungs of chickens showed that changes in intravascular pressure have little effect on either pulmonary vascular resistance (148) or capillary diameter, (149) in contrast to their marked effects on these variables in mammalian lungs. These design features may help to optimize ventilation-perfusion matching in various activities and environmental conditions.
Implications of the Avian System With Respect to Tracheal Length
Many birds have long necks and thus long tracheas. However, in about 60 different bird species, representing a variety of avian groups, the trachea is longer than the neck--sometimes very much longer. (150-152) In some species the trachea forms one or more loops or coils, which may be located low in the neck, cephalad to the sternum, in association with the furcula (wishbone), or especially in association with the keel of the sternum, either deep to it or subcutaneously. These tracheal elongations are generally confined to males, and in the few species in which they also occur in females they are much less pronounced than in the males. (150) Tracheal elongation is believed to develop after birth and in some species to continue with age, the trachea becoming longer and longer, extending and looping within the body.
Although it occurs in several species of swans, (150,152) exaggerated tracheal elongation is by no means confined to long-necked birds. For example, in one species of bird-of-paradise, a bird the size of a common flicker (which has a trachea 38 mm long), Clench (151) documented a total tracheal length of over 800 mm, which was almost as long as that of an ostrich. (153) Figure 18 illustrates some of the patterns and sizes of tracheal extensions and loops found in birds. (150)
The fact that tracheal elongations and loops occur in disparate groups of birds and take such varied forms suggests that these features have evolved multiple times and may have adaptive value. It has been proposed that the latter has to do with vocalization. (150,154) Birds that have elongated tracheas tend to have louder and lower-pitch voices than similar species that lack elongated tracheas, and this seems to be the case especially in species in which the loop lies next to the sternum. (150,154) From a series of acoustical experiments with cranes, Gaunt et al concluded that, "The tracheal coils that are embedded in the sternum serve a function analogous to the bridge of a stringed instrument, transmitting the vibrations of a tiny sound source to a large radiating surface, the sternum. The sternum then vibrates against the large internal air reservoir of the avian air sac system." (155)
Whatever their function may be, tracheal extensions and loops do not appear to interfere with ventilation or gas exchange in the birds that have them. More to the point, it is evidently no problem for the male of a particular species to have an anatomic dead space many times greater than the female of the same species.
The Sauropod Respiratory System: Current Concepts
The respiratory system of birds is unique, consisting of a set of large-volume, high-compliance posteriorly located air sacs used to force air through a low-volume, low-compliance, cross-current-perfused lung into other, more anteriorly located air sacs, resulting in nearly continuous lung ventilation and highly efficient gas exchange. Birds are also unique among living vertebrates in having extensive post-cranial skeletal pneumaticity, with extensive connections between the air-sac system and the air spaces within the bones. There is increasing evidence that this arrangement was present in the dinosaurs, (54,156,157) including the sauropods, (158-160) as well as other non-dinosaurian Mesozoic reptiles such as the flying pterosaurs. (161) Some authors have presented evidence to support the contention that dinosaurs had metabolisms and respiratory systems similar to those of crocodiles, with a tidally ventilated lung rather than the flow-through avian system, (162-166) but currently most investigators of dinosaur physiology reject that view, (167,168) particularly with respect to theropods and sauropods.
In the axial skeletons of both theropod and sauropod dinosaurs there was extensive replacement of vertebral bone with what appears to have been empty space. Evidence from isotopic and imaging studies indicates that this "empty space" represents pneumatization, just as in birds. (157,159,169-171) Figure 19 shows a cross-section through a cervical vertebra of Camarasaurus, a typical sauropod, and illustrates the relatively scant amount of solid bone it contains. (172) Figure 20 shows a similar vertebra from the neck of Diplodocus, seen in lateral view, as it appears grossly (46) and with the air-filled diverticulae added. (173) Although the pattern of bone elements differs considerably in the vertebrae of different sauropod species, much of the cervical skeleton consisted of air spaces. Wedel examined computed-tomography sections, photographs, and published images of transverse sections of the vertebrae of more than a dozen genera of sauropods and found a mean air-space proportion (the fraction of each vertebra that would have been filled with air) of 0.61 (61%), and that fraction exceeded 80% in several species. (115)
Reconstruction of the neck and thorax of Diplodocus, taking into consideration the air spaces indicated by the osteological evidence, results in a striking image, with extensive pneumatization of the axial skeleton. (45,173) Figure 21 shows the presumed avian-type lung and an anteriorly-placed air sac. However, what role--if any--the extensive skeletal pneumatization may have played in respiration is not known. Even if they did not participate in gas exchange, the skeletal air spaces would have substantially decreased body weight, especially in the neck, (115,171,174) which calls into question the traditional dinosaur-weight estimates, which were based on the assumption of approximately the same overall density as water. (175) Recently an additional hypothesis was put forth: pneumatization may also have helped directly in the support and stabilization of the neck, quite apart from any effect on respiration. (46)
The extensive air-containing spaces within the sauropod body may provide an answer to a problem previously mentioned: how to get rid of heat in such a large body. 103 It has been postulated that both the large air sacs and the many smaller air spaces involved in skeletal pneumatization played an important role in conditioning the air and cooling the venous blood. (56) The higher compliance and more efficient gas exchange, and, thus, lower minute-ventilation requirement, of the avian respiratory system, compared to that of existing reptiles and mammals, would also have reduced heat production by decreasing the work of breathing. (56) One study found that panting increases the efficiency of the avian air sac system in cooling inspired air, (176) and perhaps this also played a role in tropical environments.
Additional evidence in support of an avian-type respiratory apparatus comes from studies of skeletal movement during ventilation and the role of the uncinate processes, which are small extensions from the caudal edges of birds' vertebral ribs. Uncinate processes were once believed to be involved in locomotion, through stabilization of the shoulders, but they have now been shown to play a crucial role in respiration during both inspiration and expiration. (177,178) The identification of uncinate processes in several types of dinosaurs further strengthens the avian-dinosaur connection and the likelihood that dinosaurs had aviantype respiratory systems. Similarly, recent studies of theropod dinosaur bones and muscle attachments involved in "pelvic aspiration" (the mechanism by which birds fill their abdominal air sacs) further support the contention that they had this ventilation mechanism and an avian-type respiratory system. (179)
Figure 22, from Wedel, presents a more complete depiction of the respiratory system of a sauropod such as Diplodocus, with the pneumatic diverticulae less dramatically shown than in Figure 21. (171) Although much of this discussion has focused on sauropods because of the greater challenges posed by their bulk and long necks, there is increasing evidence that essentially all the described sauropod features were present in the theropods as well. (54,156,157)
Influence of the Ancient Atmosphere
The fluctuations in the atmospheric [O.sub.2] concentration during the last 550 million years (Fig. 23) (57) are now believed to have profoundly affected the evolution of animals. (59,62,180) A 100-million-year spike in atmospheric [O.sub.2] concentration spanning the mid-Devonian, Carboniferous, and Permian periods, during which [O.sub.2] was as high as 35%, coincided with the biggest surge in animal gigantism prior to the emergence of the dinosaurs. (181) Insects rely on direct diffusion of atmospheric oxygen into their tissues for respiration, and during the Devonian-through-Permian [O.sub.2] spike they attained sizes never seen before or since--including dragonflies with 70-cm wingspans. (181,182) Amphibians, in which a substantial proportion of total oxygen consumption is via skin-diffusion, reached lengths well in excess of 2 m--much larger than any amphibians since that time. (182,183)
Although it took perhaps 50 million years, the atmospheric [O.sub.2] concentration then fell from its zenith about 300 million years ago to its lowest level since the beginning of the Phanerozoic (10% or 11%, according to the most widely accepted model)58,60 at the end of the Permian period and the beginning of the Triassic (Fig. 23). The giant insects and amphibians had disappeared. In the hypoxic early Triassic the ancestors of both the dinosaurs and the mammals were present. However, during perhaps 50 million years with the atmospheric [O.sub.2] roughly equivalent to a present-day altitude of 4,300 m (14,000 feet), it was the dinosaurs, not the mammals, that emerged, diversified, radiated widely, and in some instances became huge. There is evidence that atmospheric [O.sub.2] gradually increased during the Jurassic, reached a percentage in the low 20s at the time of the largest sauropods, and then diminished somewhat until another, smaller [O.sub.2] spike during the mid-Tertiary, about 50 million years ago. (62) It was only during that time (after the dinosaurs had become extinct) that mammals grew larger and became the dominant land vertebrates.
It is tempting--almost inescapable--to conclude that a crucial breakthrough with respect to respiration occurred during the period of prolonged hypoxia in the Triassic, which enabled the dinosaurs to emerge and leave the early mammals behind. That breakthrough appears to have been the flow-through avian respiratory system. The inspired [O.sub.2] tension depicted in Figure 17 is about the same as that which was present 220-180 million years ago, at the time of the great dinosaur radiation. As Figure 17 shows, and as migrating birds demonstrate, the avian respiratory system works just fine in that relatively hypoxic environment. The avian system, with its greater oxygen-extraction efficiency and its freedom from the constraints of tracheal dead space, appears to have played a key role in the emergence of the dinosaurs--at least the emergence of the theropods and the giant sauropods--and to have enabled them to dominate terrestrial life on earth for more than 150 million years.
Application of physical principles that affect respiration, evidence from living long-necked animals, comparison with other vertebrate respiratory systems, exciting breakthroughs in the study of dinosaur fossils, and inference from knowledge about the ancient atmosphere permit more confident conclusions about how the dinosaurs breathed than would have been possible 2 or 3 decades ago:
* The giant sauropods could not have been amphibious, extending their long necks from under water to breathe from the surface snorkel-fashion, because the weight of the water would have made it impossible to inflate their lungs, and the pressure differential from pulmonary capillaries to alveolar air would have produced catastrophic leakage.
* It is exceedingly unlikely that a respiratory system similar to the giraffe's could have worked in the sauropods, because of excessive airway resistance, excessive dead space, or both.
* Extensive pneumatization of the post-cranial skeletons of both sauropods and theropods has been convincingly demonstrated, which strongly supports the assertion that they had avian-type respiratory systems, with air sacs and flow-through lungs.
* An avian-type respiratory system, in which dead space in even a greatly elongated trachea does not present an insurmountable impediment to ventilation or gas exchange, would explain how the giant sauropods were able to breathe with such long necks.
* The development of such a respiratory system at a time when the atmospheric [O.sub.2] concentration was much lower than today could explain why the dinosaurs were able to increase in size and dominate the terrestrial world, and why the mammals, which had the less efficient tidal ventilation system of ancestral reptiles, were not.
Writing this article in the 1970s, when I first began wondering how comparative respiratory physiology might apply to the largest dinosaurs, would have been a very different undertaking--one in which speculation would have been much less hindered by the presence of actual data. In the last few years there has been a veritable explosion of new knowledge--from new dinosaur discoveries to new types of evidence and application of new technology to increased insight into existing evidence.
The available evidence clarifies some of the issues, and though it does not definitively answer the questions posed at the beginning of this article, it permits a number of conclusions to be drawn. How to perfuse a head 12 m (40 ft) above the ground poses hydrodynamic problems without ready solutions, which encourages speculation about alternative strategies for both blood flow and hypoxia tolerance in the largest sauropods. New hypotheses about the metabolic status of these giant creatures are gaining both theoretical and empirical support. Increasing confidence in the dinosaurian ancestry of birds, and mounting evidence of an avian-type respiratory system in theropods and sauropods suggest answers to the problems of why the large tracheal dead space of their very long necks did not prevent their evolutionary success, and why dinosaurs rather than mammals came to dominate the terrestrial world during the early Mesozoic, when there was much less [O.sub.2] in the atmosphere than there is today.
Although in addressing the physiology of dinosaurs I have ventured far from the bedside and included topics that Dr Egan might not have anticipated, I have tried to be true to the intent of the lectureship bearing his name: to summarize new information in a rapidly evolving field; to increase understanding and insight in subject areas important to the foundation of respiratory care; and to stimulate and broaden the interests of those involved in this field.
I thank Erik R Swenson MD and Michael P Hlastala PhD for their helpful review of the manuscript, and Andrew D Blair Jr PhD for assistance with Figure 15.
(1.) Pierson DJ. The evolution of breathing: 1. Why comparative physiology? Respir Care 1982;27(1):51-54.
(2.) Pierson DJ. The evolution of breathing: 2. The nature of the problem. Respir Care 1982;27(2):160-163.
(3.) Pierson DJ. The evolution of breathing: 3. Viable solutions: types of respiratory apparatus in animals. Respir Care 1982;27(3):267270.
(4.) Pierson DJ. The evolution of breathing: 4. There and back again: the respiratory advantages of a circulatory system. Respir Care 1982;27(5):569-579.
(5.) Pierson DJ. The evolution of breathing: 5. Oxygen-carrying pigments: respiratory mass transit. Respir Care 1982;27(8):963-970.
(6.) Pierson DJ. The evolution of breathing: 6. Getting by with diffusion: animals with no breathing organs. Respir Care 1982;27(9): 1063-1069.
(7.) Pierson DJ. The evolution of breathing: 7. Tube feet and water lungs: the echinoderms. Respir Care 1982;27(11):1392-1396
(8.) Pierson DJ. The evolution of breathing: 8. Gill respiration in mollusks and crustaceans-variations on a theme. Respir Care 1983; 28(2):195-202.
(9.) Glut DF, Brett-Surman MK. Dinosaurs and the media. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:675-720.
(10.) Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997.
(11.) Weishampel DB, Dodson P, Osmolska H, editors. The dinosauria, 2nd edition. Berkeley: University of California Press; 2004.
(12.) Currie PJ, Padian K, editors. Encyclopedia of dinosaurs. San Diego: Academic Press; 1997.
(13.) Farlow JO. Dinosaurs and geologic time. In: Farlow JO, BrettSurman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:107-111.
(14.) Geological Society of America. 2009 Geologic time scale. http:// www.geosociety.org/science/timescale/timesel.pdf. Accessed May 13, 2009.
(15.) Schweitzer MH. Molecular paleontology: rationale and techniques for the study of ancient biomolecules. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:136-149.
(16.) Chin K, Eberth DA, Schweitzer MH, Rando TA, Sloboda WJ, Homer JR. Remarkable preservation of undigested muscle tissue within a late Cretaceous tyrannosaurid coprolite from Alberta, Canada. Palaios 2003;18(3):286-294.
(17.) Dodson P. Sauropod paleoecology. In: Weishampel DB, Dodson P, Osmolska H, editors. The dinosauria. Berkeley: University of California Press; 1990:402-407.
(18.) Tiffney BH. Land plants as food and habitat in the age of dinosaurs. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:352-370.
(19.) Prasad V, Stromberg CA, Alimohammadian H, Sahni A. Dinosaur coprolites and the early evolution of grasses and grazers. Science 2005;310(5751):1177-1180.
(20.) Fastovsky DE, Smith JB. Dinosaur paleoecology. In: Weishampel DB, Dodson P, Osmolska H, editors. The dinosauria, 2nd edition. Berkeley: University of California Press; 2004:614-626.
(21.) Hummel J, Gee CT, Sudekum KH, Sander PM, Nogge G, Clauss M. In vitro digestibility of fern and gymnosperm foliage: implications for sauropod feeding ecology and diet selection. Proc Biol Sci 2008;275(1638):1015-1021.
(22.) Chin K. What did dinosaurs eat? Coprolites and other direct evidence of dinosaur diets. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:371-382.
(23.) Hirsch KE, Zelenitsky DK. Dinosaur eggs. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:394-402.
(24.) Sato T, Cheng YN, Wu XC, Zelenitsky DK, Hsiao YF. A pair of shelled eggs inside a female dinosaur. Science 2005;308(5720): 375.
(25.) Chiappe LM, Coria RA, Dingus L, Jackson F, Chinsamy A, Fox M. Sauropod dinosaur embryos from the late Cretaceous of Patagonia. Nature 1998;396:258-261.
(26.) Chiappe LM, Jackson F, Coria RA, Dingus L. Nesting titanosaurs from Auca Mahuevo and adjacent sites. In: Curry Rogers KA, Wilson JA, editors. The sauropods: evolution and paleobiology. Berkeley: University of California Press; 2005:285-302.
(27.) Varricchio DJ, Moore JR, Erickson GM, Norell MA, Jackson FD, Borkowski JJ. Avian paternal care had dinosaur origin. Science 2008;322(5909):1826-1828.
(28.) Farlow FO, Chapman RE. The scientific study of dinosaur footprints. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:519-553.
(29.) Lockley MG. The paleoecological and paleoenvironmental utility of dinosaur tracks. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997: 554-578.
(30.) Wright JL. Steps in understanding sauropod diversity: the importance of sauropod hacks. In: Curry Rogers KA, Wilson JA, editors. The sauropods: evolution and paleobiology. Berkeley: University of California Press; 2005:252-284.
(31.) Li R, Lockley MG, Makovicky PJ, Matsukawa M, Norell MA, Harris JD, Liu M. Behavioral and faunal implications of Early Cretaceous deinonychosaur trackways from China. Naturwissenschaften 2008;95(3):185-191.
(32.) Day JJ, Norman DB, Upchurch P, Powell HP. Dinosaur locomotion from a new trackway. Nature 2002;415(6871):494-495.
(33.) Holtz TR Jr, Brett-Surman MK. The taxonomy and systematic of the dinosaurs. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:92-106.
(34.) Sereno PC. The origin and evolution of dinosaurs. Annu Rev Earth Planet Sci 1997;25:435-489.
(35.) Sereno PC. The logical basis of phylogenetic taxonomy. Syst Biol 2005;54(4):595-619.
(36.) Benton MJ. Origin and relationships of dinosauria. In: Weishampel DB, Dodson P, Osmolska H, editors. The dinosauria, 2nd edition. Berkeley: University of California Press; 2004:7-20.
(37.) Sereno PC. The evolution of dinosaurs. Science 1999;284(5423): 2137-2147.
(38.) Fiorillo AR, Eberth DA. Dinosaur taphonomy. In: Weishampel DB, Dodson P, Osmolska H, editors. The dinosauria, 2nd edition. Berkeley: University of California Press; 2004: 607-613.
(39.) Chapman RE. Technology and the study of dinosaurs. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:112-135.
(40.) Rowe T, McBride EF, Sereno PC. Dinosaur with a heart of stone. Science 2001;291(5505):783.
(41.) Alexander RM. Engineering a dinosaur. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:414-425.
(42.) Paladino FV, Spotila JR, Dodson P. A Blueprint for Giants: Modeling the Physiology of Large Dinosaurs. In: Farlow JO, BrettSurman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:491-504.
(43.) Stevens KA, Parrish MJ. Digital reconstructions of sauropod dinosaurs and implications for feeding. In: Curry Rogers KA, Wilson JA, editors. The sauropods: evolution and paleobiology. Berkeley: University of California Press; 2005:178-200.
(44.) Alexander RM. Dinosaur biomechanics. Proc Biol Sci 2006; 273(1596):1849-1855.
(45.) Sellers WI, Manning PL. Estimating dinosaur maximum running speeds using evolutionary robotics. Proc Biol Sci 2007;274(1626): 2711-2716.
(46.) Schwarz-Wings D, Frey E. Is there an option for a pneumatic stabilization of sauropod necks? -an experimental and anatomical approach. Palaeontologia Electronica 2008;11(3):17A:26p.
(47.) Bates KT, Manning PL, Hodgetts D, Sellers WI. Estimating mass properties of dinosaurs using laser imaging and 3D computer modeling. PLoS ONE 2009;4(2):e4532.
(48.) Maina JN. Comparative respiratory morphology: themes and principles in the design and construction of the gas exchangers. Anat Rec 2000.;261(1):25-44.
(49.) Maina JN. Fundamental structural aspects and features in the bioengineering of the gas exchangers: comparative perspectives. Adv Anat Embryol Cell Biol 2002;163:111-XII, 1-108.
(50.) Maina J. 8: Comparative morphology of the gas exchangers. J Anat 2002;200(2):201-202.
(51.) Perry SF, Sander M. Reconstructing the evolution of the respiratory apparatus in tetrapods. Respir Physiol Neurobiol 2004;144(2-3): 125-139.
(52.) Dzemski G, Christian A. Flexibility along the neck of the ostrich (Struthio camelus) and consequences for the reconstruction of dinosaurs with extreme neck length. J Moiphol 2007;268(8):701-714.
(53.) Hicks JW, Farmer CG. Gas exchange potential in reptilian lungs: implications for the dinosaur-avian connection. Respir Physiol 1999; 117(2-3):73-83.
(54.) O'Connor PM, Claessens LP. Basic avian pulmonary design and flow-through ventilation in non-avian theropod dinosaurs. Nature 2005;436(7048):253-256.
(55.) Maina JN. Development, structure, and function of a novel respiratory organ, the lung-air sac system of birds: to go where no other vertebrate has gone. Biol Rev Camb Philos Soc 2006;81(4):545579.
(56.) Perry SF, Christian A, Breuer T, Pajor N, Codd JR. Implications of an avian-style respiratory system for gigantism in sauropod dinosaurs. J Exp Zool Part A Ecol Genet Physiol. Feb 2 2009. [Epub ahead of print]
(57.) Berner RA. Atmospheric oxygen over Phanerozoic time. Proc Natl Acad Sci USA 1999;96(20):10955-10957.
(58.) Berner RA. The Phanerozoic carbon cycle: CO2 and O2. Oxford: Oxford University Press; 2004.
(59.) Berner RA, Vandenbrooks JM, Ward PD. Evolution. Oxygen and evolution. Science 2007;316(5824):557-558.
(60.) Berner RA. GEOCARBSULF: a combined model for Phanerozoic atmospheric O2 and CO2. Geochimica et Cosmochimica Acta 2006; 70(23):5653-5664.
(61.) Bergman NM, Lenton TM, Watson AJ. COPSE: a new model of biogeochemical cycling over Phanerozoic time. Am J Sci 2004; 304(5):397-437.
(62.) Falkowski PG, Katz ME, Milligan AJ, Fennel K, Cramer BS, Aubry MP, et al. The rise of oxygen over the past 205 million years and the evolution of large placental mammals. Science 2005;309(5744): 2202-2204.
(63.) Falkowski PG, Isozaki Y. Geology. The story of O2. Science 2008; 322(5901):540-542.
(64.) McIntosh JS, Brett-Surman MK, Farlow JO. Sauropods. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:264-290.
(65.) Upchurch P, Barrett PM, Dodson P. Sauropoda. In: Weishampel DB, Dodson P, Osmolska H, editors. The dinosauria, 2nd edition. Berkeley: University of California Press; 2004:259-322.
(66.) Curry Rogers KA, Wilson JA, editors. The sauropods: evolution and paleobiology. Berkeley: University of California Press; 2005.
(67.) Sander PM, Clauss M. Paleontology. Sauropod gigantism. Science 2008;322(5899):200-201.
(68.) Wilson JA, Curry Rogers K. Introduction: monoliths of the Mesozoic. In: Curry Rogers KA, Wilson JA, editors. The sauropods: evolution and paleobiology. Berkeley: University of California Press; 2005:1-14.
(69.) Smith JB, Lamanna MC, Lacovara KJ, Dodson P, Smith JR, Poole JC, et al. A giant sauropod dinosaur from an upper Cretaceous mangrove deposit in Egypt. Science 2001;292(5522):1704-1706.
(70.) Curry Rogers K, Erickson GM. Sauropod histology: microscopic views on the lives of giants. In: Curry Rogers KA, Wilson JA, editors. The sauropods: evolution and paleobiology. Berkeley: University of California Press; 2005:303-326.
(71.) Goetz RH, Warren JV, Gauer OH, Patterson JL, Doyle JT, Keen EN, McGregor M. Circulation of the giraffe. Circ Res 1960;8:10491058.
(72.) Van Citters RL, Kemper WS, Franklin DL. Blood pressure responses of wild giraffes studied by radio telemetry. Science 1966; 152(3720):384-386.
(73.) Hohnke LA. Haemodynamics in the Sauropoda. Nature 1973; 244(5414):309-310.
(74.) Choy DS, Altman P. The cardiovascular system of barosaurus: an educated guess. Lancet 1992;340(8818):534-536.
(75.) Bakker RT. Dinosaur feeding behavior and the origin of flowering plants. Nature 1978;274:661-663.
(76.) Millard RW, Lillywhite HB, Hargens AR. Cardiovascular system design and barosaurus (letter). Lancet 1992;340(8824):914.
(77.) Rewell RE. Cardiovascular system design and barosaurus (letter). Lancet 1992;340(8824):914.
(78.) Dennis JM. Barosaurus and its circulation (letter). Lancet 1992; 340(8829):1228.
(79.) Taylor MA. Barosaurus and its circulation (letter). Lancet 1992; 340(8829):1228.
(80.) Hicks JW, Badeer HS. Barosaurus and its circulation (letter). Lancet 1992;340(8829):1229.
(81.) Seymour RS. Dinosaurs, endothermy and blood pressure. Nature 1976;262:207-208.
(82.) Hicks JW, Badeer HS. Siphon mechanisms in collapsible tubes: application to circulation of the giraffe head. Am J Physiol 1989; 256(2 Pt 2):R567-R571.
(83.) Badeer HS. Is the flow in the giraffe's jugular vein a "free" fall? Comp Biochem Physiol A Physiol 1997;118(3):573-576.
(84.) Badeer HS, Hicks JW. Circulation to the head of barosaurus revisited: theoretical considerations. Comp Biochem Physiol A Physiol 1996;114(3):197-203.
(85.) Seymour RS, Lillywhite HB. Hearts, neck posture and metabolic intensity of sauropod dinosaurs. Proc Biol Sci 2000;267(1455): 1883-1887.
(86.) Stevens KA, Parrish JM. Neck posture and feeding habits of two Jurassic sauropods. Science 1999;284(5415):798-800.
(87.) Hochachka PW, Lutz PL. Mechanism, origin, and evolution of anoxia tolerance in animals. Comp Biochem Physiol B Biochem Mol Biol 2001;130(4):435-459.
(88.) Lutz PL, Nilsson GE. Vertebrate brains at the pilot light. Respir Physiol Neurobiol 2004;141(3):285-296.
(89.) Ramirez JM, Folkow LP, Blix AS. Hypoxia tolerance in mammals and birds: from the wilderness to the clinic. Annu Rev Physiol 2007;69:113-143.
(90.) Bickler PE, Buck LT. Hypoxia tolerance in reptiles, amphibians, and fishes: life with variable oxygen availability. Annu Rev Physiol 2007;69:145-170.
(91.) Milton SL, Prentice HM. Beyond anoxia: the physiology of metabolic downregulation and recovery in the anoxia-tolerant turtle. Comp Biochem Physiol A Mol Integr Physiol 2007;147(2):277290.
(92.) Folkow LP, Ramirez JM, Ludvigsen S, Ramirez N, Blix AS. Remarkable euronal hypoxia tolerance in the deep-diving adult hooded seal (Cystophora cristata). Neurosci Lett 2008;446(2-3):147-50.
(93.) Weber RE, Fago A. Functional adaptation and its molecular basis in vertebrate hemoglobins, neuroglobins and cytoglobins. Respir Physiol Neurobiol 2004;144(2-3):141-159.
(94.) Brunori M, Vallone B. Neuroglobin, seven years after. Cell Mol Life Sci 2007;64(10):1259-1268.
(95.) Greenberg DA, Jin K, Khan AA. Neuroglobin: an endogenous neuroprotectant. Curr Opin Pharmacol 2008;8(1):20-24.
(96.) Bakker RT. Anatomical and ecological evidence of endothermy in dinosaurs. Nature 1972;238:81-85.
(97.) Graham JB, Dickson KA. Tuna comparative physiology. J Exp Biol 2004;207(Pt 23):4015-4024.
(98.) Reid REH. How dinosaurs grew. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:403-413.
(99.) Seebacher F, Grigg GC, Beard LA. Crocodiles as dinosaurs: behavioural thermoregulation in very large ectotherms leads to high and stable body temperatures. J Exp Biol 1999;202(1):77-86.
(100.) Lehman TM, Woodward HN. Modeling growth rates for sauropod dinosaurs. Paleobiology 2008;34(2):264-281.
(101.) Sander PM. Long bone histology of the Tendaguru sauropods: implications for growth and biology. Paleobiology 2000;26:466-488.
(102.) Erickson GM, Rogers KC, Yerby SA. Dinosaurian growth patterns and rapid avian growth rates. Nature 2001;412(6845):429-433.
(103.) Gillooly JF, Allen AP, Charnov EL. Dinosaur fossils predict body temperatures. PLoS Biol 2006;4(8):e248.
(104.) Maina JN, West JB. Thin and strong! The bioengineering dilemma in the structural and functional design of the blood-gas barrier. Physiol Rev 2005;85(3):811-844.
(105.) Reid REH. Dinosaurian physiology: the case for "intermediate" dinosaurs. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:449-473.
(106.) Padian K, Horner JR. Dinosaur physiology. In: Weishampel DB, Dodson P, Osmolska H, editors. The dinosauria, 2nd edition. Berkeley: University of California Press; 2004:660-671.
(107.) Norman D. Dinosaur! New York: Prentice Hall; 1991:69-70.
(108.) Fisher PE, Russell DA, Stoskopf MK, et al. Cardiovascular evidence for an intermediate or higher metabolic rate in an ornithischian dinosaur. Science 2000;288(5465):503-505.
(109.) Czerkas SJ, Olson EC, editors. Dinosaurs past and present. Natural History Museum of Los Angeles County, and University of Washington Press; 1987.
(110.) Linda Hall Library. Paper dinosaurs 1824-1969. Exhibit, 1996, Kansas City, MO. http://www.lindahall.org/events exhib/exhibit/exhibits/dino/ index.shtml. Accessed May 13, 2009.
(111.) Lincoln Bannett. World with a difference. TIME Magazine. November 14, 1955:305. http://www.time.com/time/magazine/article/ 0,9171,823996,00.htm1. Accessed May 13, 2009.
(112.) Kermack KA. A note on the habits of sauropods. Annals and Magazine of Natural History 1951;12(4):830-832.
(113.) Alexander RM. Dynamics of dinosaurs & other extinct giants. New York: Columbia University Press; 1989:24.
(114.) Black LF, Hyatt RE. Maximal respiratory pressures: normal values and relationship to age and sex. Am Rev Respir Dis 1969;99(5): 696-702.
(115.) Wedel MJ. Postcranial pneumaticity in dinosaurs and the origin of the avian lung. PhD dissertation. Berkeley: University of California; 2007.
(116.) Hugh-Jones P, Barter CE, Hime JM, Rusbridge MM. Dead space and tidal volume of the giraffe compared with some other mammals. Respir Physiol 1978;35(1):53-58.
(117.) Salt GW. Respiratory evaporation in birds. Biol Rev 1964;39:113-136.
(118.) Schmidt-Nielsen K. How birds breathe. Sci Am 1971;225(6):72-79.
(119.) Schmidt-Nielsen K. How animals work. New York: Cambridge University Press; 1972.
(120.) Duncker HR. The lung air sac system of birds. A contribution to the functional anatomy of the respiratory apparatus. Ergeb Anat Entwicklungsgesch 1971;45(6):7-171.
(121.) Piiper J, Scheid P. Comparative physiology of respiration: functional analysis of gas exchange organs in vertebrates. Int Rev Physiol 1977;14:219-253.
(122.) Duncker HR. General morphological principles of amniotic lungs. In: Piiper J, editor. Respiratory function in birds, adult and embryonic (proceedings in life sciences). Berlin and Heidelberg: Springer-Verlag; 1978:2-15.
(123.) Brown RE, Brain JD, Wang N. The avian respiratory system: a unique model for studies of respiratory toxicosis and for monitoring air quality. Environ Health Perspect 1997;105(2):188-200.
(124.) Maina JN. The lung-air sac system of birds. Berlin: Springer; 2005.
(125.) James AE, Hutchins G, Stitik F, Natarajan TK, Steig P, Heller RM, et al. Avian respiration-how birds breathe. AJR 1975;125(4):986.
(126.) James AE, Hutchins G, Bush M, Natarajan TK, Burns B. How birds breathe: correlation of radiographic & anatomical and pathological studies. J Am Vet Rad Soc 1976;17:77-86.
(127.) Maina JN. Some recent advances on the study and understanding of the functional design of the avian lung: morphological and morphometric perspectives. Biol Rev Camb Philos Soc 2002;77(1):97152.
(128.) Powell FL. Respiration. In: Whittow GC, editor. Avian physiology, 5th edition. New York: Academic Press; 2000:233-264.
(129.) Magnussen H, Willmer H, Scheid P. Gas exchange in air sacs: contribution to respiratory gas exchange in ducks. Respir Physiol 1976;26(1):129-146.
(130.) Bretz WL, Schmidt-Nielsen K. Bird respiration: flow patterns in the duck lung. J Exp Biol 1971;54(1):103-118.
(131.) Kuethe DO. Fluid mechanical valving of air flow in bird lungs. J Exp Biol 1988;136:1-12.
(132.) Scheid P, Piiper J. Aerodynamic valving in the avian lung. Acta Anaesthesiol Scand 1989;33(Suppl 90):28-31.
(133.) Wang N, Banzett RB, Nations CS, Jenkins EA. An aerodynamic valve in the avian primary bronchus. J Exp Zool 1992;262(4):441445.
(134.) Maina JN, Africa M. Inspiratory aerodynamic valving in the avian lung: functional morphology of the extrapulmonary primary bronchus. J Exp Biol 2000;203(Pt 18):2865-2876.
(135.) Patterson JR, Russell GK, Pierson DJ, Levin DC, Nett LM, Petty TL. Evaluation of a fluidic ventilator: a new approach to mechanical ventilation. Chest 1974;66(6):706-711.
(136.) Moon RE, Bergquist LV, Conklin B, Miller JN. Monaghan 225 ventilator use under hyperbaric conditions. Chest 1986;89(6):846-851.
(137.) Weill H, Williams TB, Burk RH. Laboratory and clinical evaluation of a new volume ventilator. Chest 1975;67(1):14-19.
(138.) Klain M, Smith RB. Fluidic technology. A discussion and a description of a fluidic controlled ventilator for use with high flow oxygen techniques. Anaesthesia 1976;31(6):750-757.
(139.) Winslow RM. The role of hemoglobin oxygen affinity in oxygen transport at high altitude. Respir Physiol Neurobiol 2007;158(2-3): 121-127.
(140.) Scott GR, Milsom WK. Control of breathing and adaptation to high altitude in the bar-headed goose Am J Physiol Regul Integr Comp Physiol 2007;293(1):R379-R391.
(141.) Saunders DK, Fedde MR. Exercise performance of birds. Adv Vet Sci Comp Med 1994;38B:139-190.
(142.) Milsom WK, Scott G. Respiratory adaptations in the high flying bar-headed goose. Comparative biochemistry and physiology part C. Toxicol Pharmacol 2008;148(4):460. doi:10.1016/ j.cbpc.2008.10.047.
(143.) Schmidt-Nielsen K. Locomotion: Energy cost of swimming, running and flying. Science 1972;177:222-228.
(144.) Gill RE, Tibbitts TL, Douglas DC, Handel CM, Mulcahy DM, Gottschalck JC, et al. Extreme endurance flights by landbirds crossing the Pacific Ocean: ecological corridor rather than barrier? Proc Biol Sci2009;276(1656):447-457.
(145.) Watson RR, Fu Z, West JB. Morphometry of the extremely thin pulmonary blood-gas barrier in the chicken lung. Am J Physiol Lung Cell Mol Physiol 2007;292:L769-L777.
(146.) Duncker HR. Structure of avian lungs. Respir Physiol 1972;14(1): 44-63.
(147.) Duncker HR. Structure of the avian respiratory tract. Respir Physiol 1974;22(1-2):1-19.
(148.) West JB, Watson RR, Fu Z. Major differences in the pulmonary circulation between birds and mammals. Respir Physiol Neurobiol 2007 1;157(2-3):382-390.
(149.) Watson RR, Fu Z, West JB. Minimal distensibility of pulmonary capillaries in avian lungs compared with mammalian lungs. Respir Physiol Neurobiol 2008;160(2):208-214.
(150.) McLelland J. Larynx and trachea. In: King AS, McLelland J, editors. Form and function in birds, vol 4. New York: Academic Press; 1989:69-103.
(151.) Clench MH. Tracheal elongation in birds-of-paradise. Condor 1978; 80:423-430.
(152.) Banko WE. The trumpeter swan. North American Fauna, No. 63. Washington DC: US Department of Interior, Fish and Wildlife Service; 1960.
(153.) Hinds DS, Calder WA. Tracheal dead space in the respiration of birds. Evolution 1971;25:429-440.
(154.) Zimmer C. Calling a bluff. thanks to their long windpipes, some birds sound bigger than they are. Natural History 2000 (March): 20-22.
(155.) Gaunt AS, Gaunt SLL, Prange HD, Wasser JS. The effects of tracheal coiling on the vocalizations of cranes (Aves; Gruidae). J Comp Physiol A 1987;161(1):43-58.
(156.) Codd JR, Manning PL, Norell MA, Perry SF. Avian-like breathing mechanics in maniraptoran dinosaurs. Proc Biol Sci 2008;275(1631): 157-161.
(157.) Sereno PC, Martinez RN, Wilson JA, Varricchio DJ, Alcober OA, Larsson HC. Evidence for avian intrathoracic air sacs in a new predatory dinosaur from Argentina. PLoS ONE 2008;3(9):e3303.
(158.) Daniels CB, Pratt J. Breathing in long-necked dinosaurs: did the sauropods have bird lungs? Comp Biochem Physiol A Comp Physiol 1992;101:43-46.
(159.) Wedel MJ. Vertebral pneumaticity, air sacs, and the physiology of sauropod dinosaurs. Paleobiology 2003;29(2):243-255.
(160.) Wedel MJ. Evidence for bird-like air sacs in Saurischian dinosaurs. J Exp Zool Part A Ecol Genet Physiol2009 Feb 9 [Epub ahead of print]
(161.) Claessens LP, O'Connor PM, Unwin DM. Respiratory evolution facilitated the origin of pterosaur flight and aerial gigantism. PLoS ONE 2009;4(2):e4497.
(162.) Ruben JA, Hillenius WJ, Geist NR, Jones TD, Currie PJ, Horner JR, Espe G III. The metabolic status of some late Cretaceous dinosaurs. Science 1996;273:1204-1207.
(163.) Ruben JA, Jones TD, Geist NR, Hellenius WJ. Lung structure and ventilation in therapod dinosaurs and early birds. Science 1997; 278:1267-1270.
(164.) Ruben J, Leitch A, Hillenius W, Geist N, Jones T. New insights into the metabolic physiology of dinosaurs. In: Farlow JO, Brett-Surman MK, editors. The complete dinosaur. Bloomington: Indiana University Press; 1997:505-518.
(165.) Ruben JA, Del Sasso C, Geist NR, Hillenius WJ, Jones TD, Signore M. Pulmonary function and metabolic physiology of theropod dinosaurs. Science 1999;283(5401):514-516.
(166.) Ruben JA, Jones TD, Geist NR. Respiratory and reproductive paleophysiology of dinosaurs and early birds. Physiol Biochem Zool 2003;76(2):141-164.
(167.) Claessens L. Archosaurian respiration and the pelvic girdle aspiration breathing of crocodyliforms. Proc Biol Sci 2004;271(1547): 1461-1465.
(168.) Uriona TJ, Farmer CG. Recruitment of the diaphragmaticus, ischiopubis and other respiratory muscles to control pitch and roll in the American alligator (Alligator mississippiensis). J Exp Biol 2008; 211 (Pt 7):1141-1147.
(169.) O'Connor PM. Postcranial pneumaticity: an evaluation of soft-tissue influences on the postcranial skeleton and the reconstruction of pulmonary anatomy in archosaurs. J Moiphol 2006;267(10):1199-226.
(170.) Wedel MJ. The evolution of vertebral pneumaticity in sauropod dinosaurs. J Vertebrate Palaeo 2003;23:324-328.
(171.) Wedel MJ. Postcranial skeletal pneumaticity in sauropods and its implications for mass estimates. In: Curry Rogers KA, Wilson JA, editors. The sauropods: evolution and paleobiology. Berkeley: University of California Press; 2005:201-228.
(172.) Wedel MJ, Cifelli RL, Sanders RK. Osteology, paleobiology, and relationships of the sauropod dinosaur Sauroposeidon. Acta Palaeontologica Polonica 2000;45(4):343-388.
(173.) Schwarz D, Meyer CA, Frey D, Manz H, Lehman EH. The role of pneumatization of the axial skeleton of sauropods. A constructional morphological and evolutionary analysis. Basel Natural History Museum; 2006. http://www.nmb.bs.ch/project-sauropods.htm. Accessed May 13, 2009.
(174.) Henderson DM. Tipsy punters: sauropod dinosaur pneumaticity, buoyancy and aquatic habits. Proc Biol Sci 2004;271(Suppl 4): S180-S183.
(175.) Colbert EH. The weights of dinosaurs. American Museum Novitates 1962;2076:1-16.
(176.) Bucher TL. Oxygen consumption, ventilation and respiratory heat loss in a parrot, Bolborhynchus lineola, in relation to ambient temperature J Comp Physiol 1997;142:479-488.
(177.) Codd JR, Boggs DF, Perry SF, Carrier DR. Activity of three muscles associated with the uncinate processes of the giant Canada goose (Branta Canadensis maximus). J Exp Biol 2005;208(Pt 5): 849-857.
(178.) Tickle PG, Ennos AR, Lennox LE, Perry SR, Codd JR. Functional significance of the uncinate processes in birds. J Exp Biol 2007; 210(Pt 22):3955-3961.
(179.) Carrier DR, Farmer CG. The evolution of pelvic aspiration in archosaurs. Paleobiol 2000;26(2):271-293.
(180.) Ward PD. Out of thin air: dinosauars, birds, and the earth's ancient atmosphere. Washington DC: Joseph Henry Press; 2006:282.
(181.) Graham JB, Dudley R, Aguilar N, Gans C. Implications of the late Palaeozoic oxygen pulse for physiology and evolution. Nature 1995; 375:117-120.
(182.) Dudley R. Atmospheric oxygen, giant Paleozoic insects and the evolution of aerial locomotor performance. J Exp Biol 1998;201(Pt 8):1043-1050.
David J Pierson MD FAARC is affiliated with the Division of Pulmonary and Critical Care Medicine, Harborview Medical Center, and with the Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington, Seattle, Washington.
Dr Pierson presented a version of this paper as the 35th Donald F Egan Memorial Lecture, at the 54th International Respiratory Congress of the American Association for Respiratory Care, held December 13-16, 2008, in Anaheim, California.
The author has disclosed no conflicts of interest.
Correspondence: David J Pierson MD FAARC, 325 Ninth Avenue, Box 359762, Seattle WA 98104. E-mail: email@example.com.
Table 1. Increased Interest in Dinosaur Physiology
Since 1965, as Reflected in Index Medicus Citations
Total Citations Found
With TheseSearch Terms
Publication Interval Dinosaurs Physiology
1965-1979 11 1
1980-1989 33 4
1990-1999 73 13
2000-2008 436 213
Table 2. Hypothetical Arterial Blood Pressures in the Giant
Sauropods, Based on Measured Blood Pressures in Living
Height of Head Pressure
Species Above Heart (m) (mm Hg)
Human 0.5 120
Giraffe 2.8 280
Brachiosaurus 8.3 680
Largest sauropods ~12.6 ~1,000
* The estimated pressures for the sauropods are based on
their height and assume a perfusion pressure entering the
head of about 70 mm Hg. The heights of the largest sauropods
(see text) are speculative, given that complete skeletons
have not yet been found.
Table 3. Proposed Mechanisms for Maintaining Perfusion to the
Head in the Giant Sauropods
Circulatory function essentially By proportionality to known
the same as in mammals, pressures in humans and
with proportionally higher giraffes, [greater than or
arterial blood pressure equal to] 600 mm Hg would be
([greater than or equal to] required to raise the blood to
600 mm Hg) the required height and provide
the required cerebral
Multiple hearts in series in Lower perfusion pressure
thoracic outlet and neck required, and thus less
Siphon Lower required pressures, and
thus less ventricular mass
Neck and head maintained in Less vertical distance to be
more horizontal position overcome to perfuse brain.
Requires less pressure and
less ventricular mass.
Head raised to maximum Ability to function for short
height only intermittently periods without cerebral
and briefly. No perfusion to perfusion would require less
head when neck held fully pressure and less ventricular
Mechanism Arguments For
Circulatory function essentially Consistent with well-studied
the same as in mammals, mechanisms in living animals.
with proportionally higher Would not require any unique
arterial blood pressure features not present in living
([greater than or equal to] vertebrates.
600 mm Hg)
Multiple hearts in series in Pressures generated by individual
thoracic outlet and neck pumps would be comparable to
those in living mammals.
Siphon Closed system, requiring that
ventricle overcome only
vascular resistance, not the
weight of the entire column of
Neck and head maintained in Supported by anatomical and
more horizontal position computer modeling studies of
living animals, and by studies
of sauropod skeletons.
Head raised to maximum Tolerance of periods of anoxia by
height only intermittently diving mammals, turtles, and
and briefly. No perfusion to other vertebrates. Recent
head when neck held fully discovery of multiple
erect. mechanisms of anoxia-tolerance
Mechanism Arguments Against
Circulatory function essentially Are such pressures attainable,
the same as in mammals, and could vascular tissues
with proportionally higher withstand them? By the
arterial blood pressure law of Laplace, such
([greater than or equal to] pressures would require
600 mm Hg) hearts much larger than
those of any known
Multiple hearts in series in No known precedent in
thoracic outlet and neck vertebrates. Multiple
Siphon Pressure in closed siphon
system tends to be
negative at highest point,
preventing brain perfusion.
Collapsibility of veins.
Neck and head maintained in Sauropods would have been
more horizontal position unable to browse highest
vegetation or raise head for
Head raised to maximum Falls due to loss of
height only intermittently consciousness would
and briefly. No perfusion to probably be catastrophic.
head when neck held fully Ability to browse treetops
erect. would be constrained.
Table 4. Cardiac Dimensions and Hemodynamics in Fin Whale Versus
Variable Fin Whale Barosaurus
Mass of animal (tons) 40 40
Systemic arterial blood pressure (mm Hg) 100 700
Mass of heart as a proportion of total body 0.5 5
Mass of left ventricle (kg) 135 2,000
Internal diameter of ventricular chamber in 52 52
Left-ventricular-wall thickness (cm) 10.4 52
Proportion of resting energy expenditure 10 62
accounted for by heart (%)
(Data from Reference 85.)
Table 5. Neck Lengths in the Giant Sauropods
of Neck Length of Length of
Species Vertebrae Neck (m) Neck (ft)
Diplodocus carnegii 15 6.1 20
Barosaurus lentus 16 (?) 8.5 28
Brachiosaurus brancai 13 8.5-9.6 28.0-31.5
Mamenchisaurus hochuanensis 19 9.5 31.20
Sauroposeidon proteles * Unknown 11.5 * 37.7 *
Mamenchisaurus sinocanadorum * Unknown 12 * 39 *
Supersaurus vivianae * Unknown 13.3-16.2 * 43.6-52.2 *
Amphicoelias fragillimus * Unknown 11.7-20.8 * 38.4-68.2 *
* No complete specimens have been found; these lengths are
(Data from Reference 115.)Source Citation:Pierson, David J. "The physiology of Dinosaurs: circulatory and respiratory function in the largest animals ever to walk the earth.(Donald F Egan Scientific Memorial Lecture)(Report)." Respiratory Care 54.7 (July 2009): 887(25). Academic OneFile. Gale. BROWARD COUNTY LIBRARY. 12 Aug. 2009
Gale Document Number:A203482425
Disclaimer:This information is not a tool for self-diagnosis or a substitute for professional care.
(Album / Profile) http://www.facebook.com/album.php?aid=10031&id=1661531726&l=cf90f7df9c